Preparation For Electron Microscopy

The method of preparation for EM depends on whether the objective of the investigation is to study the cell interior (cell organelles, etc.) or the morphology of the siliceous cell wall components. Only the latter is relevant to this chapter.

For a few species (usually the more heavily silicified ones) intact frustules may be obtained by simply air drying a drop of raw material after thorough rinsing with distilled water. Although delicate specimens will be crushed by

TABLE 81 Embedding for Light Microscopy

Media Refractive index Solvent Manufacturer

TABLE 81 Embedding for Light Microscopy

Media Refractive index Solvent Manufacturer

Clearax

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