Methodology

Detailed procedures for collection, preservation, and examination of phyto-plankton have been described in the UNESCO Phytoplankton manual (Sournia, 1978). Because of the wide range of habitats, cell sizes, degree of silicification, and fineness, a variety of methods are required for collection, preparation, and microscopy of diatoms. The current diatom literature contains a multitude of methods, particularly for cleaning and mounting (e.g., Ricard, 1987; Round et al., 1990). The selection mentioned below is intended as a guide and represents simple, general methods to obtain material adequate for identification at the species level.

COLLECTION AND CONCENTRATION (Table 78)

Sampling by water bottle is the recommended method (Sournia, 1978, p. 33) to obtain a correct picture of the quantitative composition of the phyto-

TABLE 78 Collection

Method

Use

Disadvantage

Water bottle

Enumeration of cells; all species

Few rare species

Pump

Quantitative and qualitative;

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