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Fig. 7.5 Effects of chilling on H2O2 accumulation in wild-type and chsl, chs2, and chs3 mutants. Plants were grown for 2 weeks at 22°C and afterwards transferred to chilling temperatures of 13 and 4°C. Accumulation of hydrogen peroxide was indicated by DAB staining after 7 days at chilling conditions

Fig. 7.5 Effects of chilling on H2O2 accumulation in wild-type and chsl, chs2, and chs3 mutants. Plants were grown for 2 weeks at 22°C and afterwards transferred to chilling temperatures of 13 and 4°C. Accumulation of hydrogen peroxide was indicated by DAB staining after 7 days at chilling conditions

(2) chs2 - was mapped to chromosome 4 in the region between 9.02 and 9.95 Mb.

(3) chs3 - was mapped to chromosome 5 in the region between 5.41 and 7.37 Mb.

This initial mapping of the chsl, chs2, and chs3 genes to a final resolution of approximately 1 Mb will facilitate in the future the final cloning and isolation of these important genes that are crucial for survival of Arabidopsis plant at low chilling temperatures. As indicated in Sect. 3, the chs3 gene was recently mapped to the top of chromosome 5 and its sequence was identified (Yang et al. 2010).

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