Figure 2. Fructan concentration for each independent line was determined from seven individual seeds (dark bars) containing the vacuole-targeted SacB gene. Polymer was not detected in mature wild-type (WT) seeds. Seeds were harvested at 45 - 50 DPP and dried, prior to analysis (dry weight, dw). Only the transgenic line 250.73 was homozygous for SacB. The number of intact SacB gene copies varied among independent transformed lines. Redrawn from Caimi et al. Plant Physiol. 110: 355-363 (1996) with permission.
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