To explore the molecular mechanisms associated with the loss of seed vigour during accelerated ageing, a differential proteomic approach was used. To achieve this, total soluble proteins extracted from all seed lots were separated by 2D-PAGE. Following silver nitrate staining, protein patterns were analysed by image analysis. A typical gel is presented in Fig. 34.1. We first investigated the effect of the CDT on the dry seed proteome (i.e. from the proteome of seeds collected after conducting the treatment). Only a few protein spots showed reproducible variations in their accumulation level (Fig. 34.1).
Our differential proteomic approach reveals that 12 protein spots were more abundant in the aged seeds and six protein spots were less abundant in the deteriorated seeds. These results highlight that protein modifications can occur during artificial seed ageing.
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