A. fatua seeds were hand-collected from six locations in the northern wheat belt of Australia (i.e. from near Springsure, Toowoomba and Warwick in Queensland, and near Narrabri, Coonabarabran and Moree in New South Wales). The collected seeds were used to generate six near isogenic lines (one per collection; Armstrong, 1994) using the single seed desent method. In this procedure, each line underwent at least three generations of self-pollination to produce partially homozygous (at least 88% homozygous; Allard, 1960) lines.
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