Results and Discussion

In order to test the hypothesis that priming changes the expression of genes that confer cross-tolerance to the seeds and subsequent seedlings, we first wanted to confirm Fig. 14.1. Effect of hydrogen peroxide (H2O2) concentration on the mean germination percentages of non-treated (0) and osmoprimed ( ) tomato seeds. Germinations were performed with four replicates of 50 seeds. Bars represent standard error (se). that priming induces cross-tolerance. Therefore, we tested the effect of...

Proteome variation of germinating seeds

The proteome of the artificially aged seed lots was also analysed after 1 day imbibition in water. This stage corresponds to the germination sensu stricto of Arabidopsis (Ler) non-deteriorated seeds (none of the seeds showed radicle protrusion at that time see Table 34.1). This analysis revealed the proteome evolution of differentially aged seeds during imbibition. Among 54 protein spots presenting reproducible variations in their accumulation level, 34 were less abundant in germinating...

Results and Discussion Lettuce

The vigour assessment system was used to numerically evaluate four lettuce seed lots, with different levels of seed vigour, 3 days after the initiation of germination (Fig. 32.1). The software marked hypocotyls in red and radicles in green. For most seedlings, these features were detected correctly. For the purpose of this study, lettuce was selected as a model species because its seeds typically produce seedlings that are straight with only one primary root. Moreover, lettuce has seedling...

Materials and Methods

Plant material and germination experiments Germination assays were carried out on four replicates of 100 sugarbeet seeds (KWS, Einbeck, Germany) and repeated in at least three independent experiments. Germination characteristics were evaluated in a pleated filter paper assay according to International Seed Testing Association (ISTA) regulations (ISTA, 1985) with 30 ml H2O at 15 C. Germination was followed by daily counts, and final germination was determined after 14 days. A seed was considered...

Materials and Methods Plant material

The Arabidopsis wild-type (WT Columbia ecotype) and transgenic plants were grown in a greenhouse under long-day conditions (i.e. in a 16 h light 8 h dark cycle) in pots containing a 1 3 vermiculite soil mixture. Sterilized seeds were plated on MS medium (Murashige and Skoog, 1962) containing 1 (w v) sucrose and solidified with 1 (w v) agar for in vitro culture. Vector construction and plant transformation To analyse the function of FsPP2C2 in the regulation of dormancy and germination by ABA,...

Characterization of oxidized proteins in the dry mature seeds

Ageing in all organisms, notably in plants, is associated with oxidative stress (Bailly, 2004) that entails oxidation, by carbonylation, of specific proteins (Berlett and Stadtman, 1997 Toda, 2000). Since highly sensitive methods have been described Table 34.1. Germination parameters of Arabidopsis seed lots subjected to different periods of controlled deterioration treatment (CDT). Table 34.1. Germination parameters of Arabidopsis seed lots subjected to different periods of controlled...

Seed Germination References

Adachi, M., Takenaka, Y., Gidamis, A.B., Mikami, B. and Utsumi, S. (2001) Crystal structure of soybean proglycinin A1aB1b homotrimer. Journal of Molecular Biology 305, 291-305. Adachi, M., Kanamori, J., Masuda, T., Yagasaki, K., Kitamura, K., Mikami, B. and Utsumi, S. (2003) Crystal structure of soybean 11S globulin Glycinin A3B4 homohexamer. Proceedings of the National Academy of Sciences of the United States of America 100, 7395-7400. Artschwager, E. (1927) Development of flowers and seed in...

MiRNAs do Exist in Seeds Now What

MiRNAs have already been shown to play critical roles in plant development. The identification of miRNAs expressed in seeds, by itself, does not provide much information towards a better understanding of the biology of seed development Table 37.1. Useful web sites for miRNA research. Memorial Sloan-Kettering Cancer Center, Computational Biology Center RNAi elegansNet Sanger Institute miRBase Arabidopsis Small RNA Project Bartel's Lab Homepage The McManus Lab Web site address http...

Free Radicals Active Oxygen Species and Antioxidants

The highly reactive free radicals, along with hydrogen peroxide H2O2 , are collectively termed active reactive oxygen species AOS ROS . While the intracellular glassy state must curtail molecular and AOS mobility, and thus interaction, during the process of desiccation, intracellular structures are highly vulnerable as conditions for radical generation are enhanced Vertucci and Farrant, 1995 Pammenter and Berjak, 1999 Walters et al., 2005a . The most frequently discussed AOS in the context of...

Results and Discussion Imbibition patterns in honey locust seeds

Scarified honey locust seeds showed a typical triphasic increase in seed size that was indicative of water uptake during imbibition Figs 33.1 and 33.2 . Acid-scarified seeds had a faster imbibition rate compared with hot water-treated seeds. Acid-treated Fig. 33.1. Increase in seed size in honey locust seeds treated with acid for 2 h or hot water for 1 min. a is initial seed size b is seed shape after 50 imbibition and indicates the micropylar end of the seed. Fig. 33.2. Pattern of seed size...