It was hypothesized that pollen possess some protective mechanism against microbial attack. Antibacterial activity is determined by disc diffusion method. The disc diffusion assay (Rasoanaivo and Urverg 1993) is used to determine the growth inhibition of bacteria by the pollen extracts.
The plates are evaluated after incubation at 37°C for 18 hours. Antibacterial activity is expressed as the ratio of the inhibition zone (mm) produced by the pollen extract and the inhibition zone caused by the reference, (Vlietinck et al., 1995).
The experimental work was carried out by Pannicker (2003) to assess the antibacterial properties of Prosopis juliflora. It was found that their pollen extracts inhibited the growth of bacteria such as Staphylococcus aureus (standard strain) and Staphylococcus aureus [MRSA], Staphylococcus aureus [VRSA] and Escherichia coli.
Further it was determined that the aqueous extracts and all other extracts of Prosopis pollen can produce reactive zone against Staphylococcus aureus [MRSA], in which commercial antibiotic has no effect, Prosopis pollen extracts thus gave better results than those produced by antibiotics like Netromycin and Vancomycin.
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