MODIFIED THIERY TEST
The modified (short) Thiery test (WEBER and FROSCH 1995) is especially effective after fixation of specimens with osmium and potassium ferrocyanide and is a good method for general enhancement of contrast in the cytoplasm and the pollen wall.
Sections 80-100 nm thick are collected on gold grids and stained with 1 % periodic acid (PA) for 10 min, 0.2 % thiocarbohydrazide (TCH) for 15 min, and 1 % silver proteinate (SP) for 10 min (all steps at room temperature). After the staining steps with PA and SP, sections are intensively washed in distilled water; after staining with TCH, washes are in 3 % acetic acid followed by distilled water.
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