Organelle Proteomics

20 CELL WALL 293

Elisabeth Jamet, Hervé Canut, Cécile Albenne, Georges Boudart, and Rafael Pont-Lezica

20.1 Introduction 293

20.2 Brief Bibliographic Review 296

20.3 Specific Methodology and Strategies 296

20.4 Experimental Results and Applications 300

20.5 Conclusions 304

20.6 Five-Year Viewpoint 304 References 306

21 PLASMA MEMBRANE: A PECULIAR STATUS AMONG THE CELL MEMBRANE SYSTEMS 309

Genevieve Ephritikhine, Anne Marmagne, Thierry Meinnel, and Myriam Ferro

21.1 Introduction and Brief Bibliographic Review 309

21.2 Specific Methodologies and Strategies (Box 21.1) 310

21.3 Experimental Results and Applications: Toward an Informative Database of the Plant PM Proteome 319

21.4 Conclusions 323

21.5 Five-Year Viewpoint 324 References 325

22 NUCLEUS 327

Subhra Chakraborty, Aarti Pandey, Asis Datta, and Niranjan Chakraborty

22.1 Introduction and Brief Bibliographic Review 327

22.2 Methodology and Strategy 328

22.3 Experimental Results and Discussion 330

22.4 Conclusions 335

22.5 Five-Year Viewpoint 336 References 337

23 CHLOROPLAST 339

Thomas Kieselbach and Wolfgang P. Schroder

23.1 Introduction 339

23.2 Brief Bibliographic Review 341

23.3 Methodology and Strategy 341

23.4 Experimental Results and Applications 344

23.5 Conclusions 348

23.6 Five-Year Viewpoint 348 References 349

24 ETIOPLAST 351

Anne von Zychlinski, Sonja Reiland, Wilhelm Gruissem, and Sacha Baginsky

24.1 Introduction 351

24.2 Etioplast Proteome Analysis 352

24.3 Conclusions 358

24.4 Five-Year Viewpoint 359 References 359

25 THE PLANT MITOCHONDRIAL PROTEOME AND THE

CHALLENGE OF HYDROPHOBIC PROTEIN ANALYSIS 361

Yew-Foon Tan and A. Harvey Millar

25.1 Introduction 361

25.2 Brief Bibliographic Review 363

25.3 Methodology and Strategy 364

25.4 Experimental Results and Applications 369

25.5 Conclusions 373

25.6 Five-Year Viewpoint 373 References 374

26 PEROXISOME 377

Yuko Arai, Youichiro Fukao, Makoto Hayashi, and Mikio Nishimura

26.1 Introduction 377

26.2 Brief Bibliographic Review 379

26.3 Specific Methodologies and Strategies 379

26.4 Experimental Results and Applications 383

26.5 Conclusions 386

26.6 Five-Year Viewpoint 387 References 388

27 UNRAVELING PLANT VACUOLES BY PROTEOMICS 391

Songqin Pan and Natasha Raikhel

27.1 Introduction 391

27.2 Bibliographic Review: Role of Proteomics in Understanding

Biology of Plant Vacuoles 393

27.3 Methodology and Strategies 395

27.4 A Case-Study of Label-Free Quantitative Proteomics 401

27.5 Conclusions 402

27.6 Five-Year Viewpoint 403 References 404

28 OIL BODIES 407

Pascale Jolivet, Luc Negroni, Sabine d'Andréa, and Thierry Chardot

28.1 Introduction and Brief Bibliographic Review 407

28.2 Methodology and Strategy 408

28.3 Conclusions 413

28.4 Five-Year Viewpoint 414 References 416

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