1-specificity (FNF)

FIGURE 8.3. FROC curves were generated in order to evaluate the various filtering options available in Progenesis 240 following analysis with the SameSpots software. A range of values for minimum spot volume, peak height, and spot radius were used. The diagonal line represents no discrimination, and the ''X'' mark in the upper left corner represents the ideal result achieved through manual spot editing. None of the filters were particularly effective in filtering out artifacts (FP) from true spots (TP), although spot volume appeared to offer slightly better selection criteria.

Regardless of which method you choose, your ideal value will be located in the upper left corner of your graph (Figure 8.3).

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