Genome Wide and Tyr Phosphoproteome Analysis of Plant STY Kinases

In order to address the question whether bona fide PTKs exist in plants, a genomic survey of protein Tyr kinase motifs in plants was performed using delineated Tyr phosphorylation motifs from the animal system [39]. In particular, plant proteomes were scanned to identify plant sequences containing a consensus that is conserved in subdomain XI of a number of biochemically characterized PTKs from mammals, Drosophila and Dictyostelium. After elimination of redundancy and FPs, sequences were scanned for the presence of motifs specific to other protein kinase subdomains; the results of this scanning (see Bioinformatic Identification of Proteomic Complements) suggested naming identified sequences as STY kinases [39]. Phylogenetic and domain architecture analysis allowed authors to cluster STY kinases into families and to infer possible involvement in signaling pathways, while hydropathy plots were used to predict subcellular localization. Finally, homology modeling of the STY kinases was performed, and on-line data from Arabidopsis microarray experiments allowed for grouping of STY genes based on their expression during a specific growth stage in a particular organ or following an environmental stimulus [39].

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