The identified proteins were sorted into various categories (Figure 22.3), according to their putative functions. However, the classification of proteins is only tentative because the biological function of many proteins identified has not yet been established experimentally. It is known that the same protein may have different functions in different subcellular compartments and can act as "moonlighting proteins," as is the case with the glycolytic pathway enzymes, GAPDH and PGK . In a number of cases, the same protein was found in multiple spots in the same gel, suggesting possible alternate PTMs. Interestingly, the relative positions of a single protein on the 2D gel indicated that the modifications affected the pI, the MW, or both. About 11% of the identified proteins were grouped under the unknown category because no information as to their potential function in the organelle was available. Other functional categories identified are explained below.
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