Contributors

Ganesh Kumar Agrawal, Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal Cecile Albenne, Surface Cellular and Signalization for Plants, Projects in Plant Biotechnology, UMR, CNRS, Paul Sabatier-Toulouse III University, Castanet-Tolosan, France Shakir Ali, Department of Biochemistry, Hamdard University, Hamdard Nagar, New Delhi, India Susan B. Altenbach, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany,...

Bioinformatics and a Leadership Role for India

In recent years, India has utilized its strength in information technology and bioinfor-matics to contribute to various genome annotation projects. For example, even though India's involvement in genome sequencing projects has been very limited, Indian scientists have participated in the sequencing of rice as well as E. histolytica genomes 90, 91 . Therefore it is obvious that the knowledge base necessary for proteome research as well as in the analysis of the data generated using information...

Oleosins and Allergy

Plant seeds and tree nuts are responsible for the vast majority of food allergies. The prevalence of peanut and tree nut allergy has doubled among the children in the United States from 1997 to 2002, reaching 1.2 75 . Considerable research has been conducted to identify and characterize nut and seed allergens 76 . Most of these allergens belong to the cupin and the prolamin superfamilies, but a new family, oleosin, has recently been identified 77 . The first suspicion of oleosin involvement in...

Methodology And Strategy Bnpage

In many respects, the practicalities of BN-PAGE are similar to a standard SDS-PAGE experiment. However, there are some features that clearly distinguish these two systems from each other. The main feature is the total absence of SDS because of its high solubilization and denaturing power. In normal SDS-PAGE, SDS is involved in the solubilization and denaturation of proteins and in the attachment of negative charges to them. Because a denaturation of the sample is not desired in BN-PAGE,...

Differential Proteome Profiles during PMIs

In rice, it is likely that there are many reports for defense proteome in response to bacteria and fungus compared to the other crop plants due to the complete sequence information of rice genome. Rice blast fungus, M. grisea, is a causal agent of rice blast disease, one of the most serious pathogens in rice plant (O. sativa L.) in most rice-growing regions of the world. By the use of 2-DGE, protein profiles of the rice leaf and suspension cell after inoculation of M. grisea were...

References

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