TRNA processing

Production of a mature functional tRNA requires several posttranscriptional processes that include maturation of the 5' and 3' extremities, nucleotide modifications including C to U editing in some cases and addition of the 3' terminal CCA nucleotides that are not genomically encoded in plant mitochondria. Several of these processes have been investigated in detail using efficient in vitro maturation systems. Plant mitochondrial trn genes are transcribed either as a single unit or as clusters...