Genetic tools to mark microorganisms with auto fluorescent proteins

A requirement of the application of AFPs is that the genes encoding these proteins have to be transformed into the bacteria. This requirement can prevent the use of GFP as a marker when a certain bacterial species or strain is not genetically accessible with the available transformation protocols. AFP genes are usually delivered on plasmids or transposons (Bloemberg et al. 2004). The advantage of a plasmid is that it is present in multiple copies, which can improve the production of AFPs and...

ACC deaminase lowering of stress ethylene

A model was previously proposed by which plant growth-promoting bacteria can lower plant ethylene levels and in turn facilitate plant growth Glick et al. 1998 . In this model the plant growth-promoting bacteria bind to the surface of a plant usually seeds or roots, although ACC deaminase-producing bacteria may also be found on leaves and flowers . In response to tryptophan and other small molecules in the plant exudates, the bacteria synthesize and secrete indole-3-acetic acid IAA , some of...

Signals affecting biocontrol factor expression at a transcriptional level

Control of root diseases by biocontrol pseudomonads involves a blend of complementary mechanisms, the most prominent being antibiosis towards plant pathogens, degradation of virulence factors produced by pathogens and induction of defence mechanisms in host plants. Efficient competition for colonization sites and micro- and macro-nutrients in the rhizo-sphere is an important prerequisite for effective biocontrol van Loon et al. 1998 Lugtenberg et al. 2001 Compant et al. 2005 Haas and Defago...