Reverse Genetics

In the absence of a good forward genetic screen, researchers have relied on reverse genetics of AtTOR, its known binding partner AtRaptor1B and presumed binding partner AtRaptor1A, and on putative downstream effectors and an upstream regulator. This work was done using the insertionally muta-genized, insertion end-mapped Arabidopsis collections (Sussman et al. 2000; Alonso et al. 2003), and elsewhere, and using homologous recombination mediated gene targeting, a promising technique in Physcomitrella (Kamisugi et al. 2006).

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