Etiology And Pathophysiology

The etiology of ASD is currently unknown, although numerous possibilities, based on pathologic findings made in ASD, are currently under investigation. Medical conditions associated with autism include epilepsy, tuberous sclerosis, blindness, deafness, and neurofibromatosis.

Genetics

Evidence suggests that ASD is highly heritable, with some estimates of its heritability reaching 90%.5 However, twin studies and the wide range of its phenotypic expression indicate that environmental influences also play an important role in the occurrence of ASD. Research has shown that monozygotic twins have a concordance of more than 60% for classical autism, without any concordance for dizygotic twins. When this same study included a broader range of cases with diagnoses of PDD, the concordance for monozygotic twins rose to 92% while that for dizygotic twins reached 10%.1 Studies also show a frequency of 2%-8% of autism in siblings of autistic children, which far exceeds the frequency of the condition in the general population.1

Oxidative stress from oxygen-bearing free radicals may have a causal role in autistic spectrum disorders.

Genetic screening studies suggest that at least 10 genes may be involved in the etiology of autism, with studies now examining numerous genes for this possibility, such as those in the speech and language region of the human genome at 7q31-q33, 15q11-q13, FOXP2, RAY1=ST7, and IMMP2L; RELN genes at 7q22-q33; a subunit of the gene for the gamma-amino-butyric acid (GABA)(A) receptor; UBE3A genes on chromosome 15q11-q13; the gene for the serotonin transporter (SERT) at 17q11-q12; and the gene for the oxytocin receptor at 3p25-p26.1 Chromosome 15 duplications are frequently examined owing to their correlation with variable degrees of language delay, ataxia, epilepsy, mental retardation, and facial abnormalities. Studies also indicate that a low level of melatonin, as the result of a primary deficit in ASMT, the gene that encodes the last enzyme in the metabolic pathway leading to melatonin synthesis, is a risk factor for ASD.6

Parental Influences and Perinatal Development

Studies have found a greater frequency of certain traits and characteristics among parents of autistic than of healthy children. Greater maternal and paternal age are independently associated with an increased risk for ASD in offspring.7 Additionally, mothers who have allergies and asthma during the second trimester of pregnancy have a twofold greater than average risk of having a child with ASD. Although this same study found maternal psoriasis to be the only autoimmune disease associated with an increased risk for ASD,8 another study found a link between both maternal ulcerative colitis and paternal type 1 diabetes and increased risk for infantile autism.9

It has been suggested that in many cases, the cerebral developmental abnormalities in autism occur before 30 weeks' gestation.10 A study of perinatal risk factors and autism found that daily maternal smoking during early pregnancy, a small birth size for gestational age, maternal birth outside of Europe or North America, congenital malformations, cesarean delivery, and a 5-minute Apgar score below 7 were all associated with an increased risk of autism.11 Another study found a link between autism and parental histories of schizophrenia-like psychosis and affective disorders,12 while yet another study found a twofold greater risk for autism among children born to mothers with diagnosed psychiatric disorders.13 The risk for autism has also been found to increase with an increasingly urban location of birth.13

Neurologic Dysfunction

Research indicates a significant generalized increase in cerebral cortical volumes of both white and gray matter in autism. This is accompanied by a normal head circumference at birth followed by an abnormally increased growth rate beginning at 12 months of age.14 Other studies have found an increased cerebellar volume and bilateral enlargement of the amygdala and hippocampus in children with ASD.15 Recent work with functional magnetic resonance imaging has demonstrated cortical neural under-connectivity in individuals with autism, as well as a smaller corpus callosum—a structure through which many bilaterally activated cortical areas communicate—in individuals with autism as compared to controls.16 Although the relevance of this is unknown, another study found greater frequencies of left-handedness and preferential left-eye use in children with autism as compared with normal groups, as well as left nasal dominance in most children with autism.17

Gastrointestinal Dysfunction

Gastrointestinal (GI) symptoms are common in children with ASD. One study found that 70% of children with ASD had histories of such symptoms, as compared to only 28% of children showing normal development.18 The most common GI symptom in this study was an abnormal stool pattern.18 Another study of GI complaints in children with ASD found that the most common complaints were chronic diarrhea, gas, and abdominal discomfort and distension.19 Reflux esophagitis was evident in over 69% of the children, chronic gastritis in 41%, and chronic duodenitis in 66%. Additionally, 58% of the children had decreased intestinal digestive enzyme activity for carbohydrate, and 75% showed increased pancreaticobiliary fluid output after intravenous administration of secretin.19 Abnormalities of the gut flora have also been seen in children with ASD, with one study finding an increased incidence of Clostridium histolyticum, a known toxin-producing organism.20

Endocrine and Metabolic Dysfunction

Numerous studies indicate endocrine and metabolic abnormalities in children with ASD. Researchers have shown that some of these children have levels of serum=plasma dehydroepiandrosterone and serum total testosterone that are significantly above age- and gender-appropriate values.21 The children with ASD were also found to have significantly subnormal levels of serum follicle-stimulating hormone, plasma-reduced glutathione, plasma cysteine, plasma methionine, and serum cystathionine. The investigators who conducted this study suggested that these findings indicate that in some children with ASD, there is an interaction between the mechanisms for trans-sulfuration in the methionine cycle that is important for detoxification and androgen pathways, causing hyper-androgenic behavior and developmental abnormalities.21 Another study found significantly lower levels of free and total carnitine and pyruvate and elevated levels of ammonia and alanine in children with ASD, possibly reflecting mild mitochondrial dysfunction.22

Oxidative Stress

Oxidative stress from oxygen-bearing free radicals may have a causal role in ASD. Individuals with autism have shown increased nitric oxide in their red blood cells and increased activity of the antioxidant enzyme glutathione peroxidase in these individuals' plasma.23 Other studies have shown increased levels of such antioxidant enzymes as superoxide dismutase and xanthine oxidase in autism.24

Increased lipid peroxidation has also been observed in individuals with autism. Transferrin, an iron-binding protein, and ceruloplasmin, a copper-binding protein, which exhibit antioxidant activity, have been found to be significantly decreased in autistic children, a finding that has been linked to a loss of language skills.25 Some children with autism show elevated levels of total homocysteine, a finding strongly correlated with decreased erythrocyte glutathione peroxidase activity and diminished levels of vitamin B12.26

Immunologic Dysfunction

Studies have indicated that children with ASD have numerous abnormalities in immune function. Studies have shown that these children have significantly increased proportion of CD4 and CD8 lymphocytes making IL-4, with a relative decrease of CD4 and CD8 cells making IL-2 and interferon-gamma indicating impaired cellular immunity and an imbalance of Th1=Th2 cytokines.27 Studies of immunoglobin (Ig) levels in children with ASD have found a significant increase in total serum protein, and serum IgG, IgG2, and IgG4, and have demonstrated a positive correlation between total serum protein and serum gamma globulin and social problems.28 Recent studies have identified serum antibodies specific for prenatally expressed brain antigens in mothers of autistic children, suggesting that these auto-antibodies could cross the placental barrier and affect fetal brain development.29 Other immunologic findings are that children with autism have significantly higher levels of antibodies to measles virus than do controls, with these antibodies present in 83% of the autistic children in one study, but not in healthy children or in the siblings of the autistic children.30 Antibodies to mumps and rubella are not elevated.30

Some autistic children exhibit auto-antibodies to brain proteins such as myelin basic protein (anti-MBP) and neuron-axon filament protein (anti-NAFP). One study found a correlation between these auto-antibodies and antibodies directed against measles virus (measles-IgG) and human herpes virus-6 (HHV-6-IgG). This study found that serum that was positive for measles-IgG and HHV-6-IgG also contained anti-MBP and anti-NAFP antibodies, suggesting virus-induced autoimmunity as a possible etiology for autism.31 Congenital rubella infection has also been linked to autism, behavioral disorders, and mental retardation.32

It is highly controversial whether measles-mumps-rubella (MMR) vaccine is a causal factor in autism, and the vast majority of studies do not support such a link.33 The possibility is based on the correlation of a type of regressive autism with concomitant GI symptoms soon after administration of the MMR vaccine.34 The finding of measles virus persistent in the intestines of some autistic children, as well as in individuals with Crohn's disease and ulcerative colitis, implies an autoimmune component in autism.34

Studies support the theory that children with autism have an impaired ability to excrete heavy metals.

Mercury, Heavy Metals, and Vaccines

Disputed epidemiologic evidence has correlated neurodevelopmental disorders with increasing doses of mercury from the ethylmercury-containing preservative thimerosal in vaccines.35 On the basis of the mercury content of the respective vaccines, children who received diphtheria-tetanus-pertussis vaccine (DTP) and Haemophilus influenza type b (Hib) vaccine at the standard vaccination schedule of 2,4,6, and 15-18 months of age may be exposed to 100 mg of mercury more than children given the combined DTP=Hib (DTPH) vaccine. According to the Vaccine Adverse Event Reporting System, this may indicate that children given the DTP and Hib vaccines as separate entities are at significantly greater risk for autism and related disorders than those given the combined vaccine.36 Thimerosal has since been removed from most vaccines.

Several studies support the theory that children with autism have an impaired ability to excrete heavy metals.37-39 One such study found significantly lower concentrations of arsenic, cadmium, and lead in the hair of such children than in controls, suggesting lower excretion rates for these three metals.37 It has also been found that children with ASD have a 2.1-fold greater than average concentration of mercury in their baby teeth, which is a good measure of cumulative exposure to toxic metals during fetal development and infancy.38 In this study, it was noted that these children had also received significantly more oral antibiotics than average during their first 12 months of life, which may have reduced the children's ability to excrete mercury, since studies with rats have found that antibiotics can almost completely inhibit the excretion of mercury by altering the gut flora.38 A study in which the levels of mercury in first childhood haircut samples from children with autism were much lower than those of controls also found that the mothers of the autistic children had had significantly greater exposure to mercury from amalgam fillings and thimerosal-containing Rho D immunoglobulin injections than did a control group.39 A Texas study found a 43% increase in the frequency of special education services and a 61% increase in the rate of autism for every 1,000 lb of environmentally released mercury.40

Serotonin

Numerous cursory studies indicate abnormalities in the metabolism of serotonin and measurable levels of this amine in autistic individuals. Among findings in this area are that platelet levels of serotonin are significantly higher in autistic than in control subjects, and that a correlation exists between platelet levels of this amine and abnormal speech development.41 Significantly lower plasma levels of serotonin have been found in mothers of autistic children than in mothers of children with normal development.42 Another finding has been that that supplementation with 5-hydroxytryptophan (5-HT), a metabolic precursor of serotonin, raised serotonin levels to a significantly greater degree in subjects with autism than in a control group.43 Abnormalities in the genes encoding the serotonin transporter and enzymes required for serotonin synthesis are subjects of current study in connection with autism.

Funny Wiring Autism

Funny Wiring Autism

Autism is a developmental disorder that manifests itself in early childhood and affects the functioning of the brain, primarily in the areas of social interaction and communication. Children with autism look like other children but do not play or behave like other children. They must struggle daily to cope and connect with the world around them.

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