Mycorrhizae and Coupling Beneficial Organisms Complete Package for Organic Systems

One key biotechnological goal is to use combined inoculation of selected rhizospheric micro-organisms to minimize fertilizer application and to maximize plant growth and nutrition (Linderman 1986; Barea and Jeffries 1995; Barea et al. 1998; Probanza et al. 2001). Literature reports confirm that selected combinations of microbial inocula enhance the positive effect

Media stub Glomus intraradices spores + roots

Mother stub Mycelia

Daughter spores

Source of G intraradices and transformed root culture (Box size 10 X 7 X 6 cm)

Media stub Glomus intraradices spores + roots

Mother stub Mycelia

Daughter spores

fttridish with germinating spores of G margarita

- G intraradices and transformed roots

ZcmeB

Zone of maximum sporulation of G. margarita

Zone of maximum sporulation of G intraradices

Fig. 5. Co-culture development process for multiple AM fungal cultivation (Tiwari P, Adho-leya A 2002)

Aerial roots

Daughter spores of G margarita

Mother stub of G. intraradices Mothers spores of G margarita Mycelia of G. margarita

Mycelia of G intraradices Daughter spores of G. intraradices

Co-culture system

Fig. 5. Co-culture development process for multiple AM fungal cultivation (Tiwari P, Adho-leya A 2002)

achieved by each microbial group considered separately (Toro et al. 1997, 1998). Also, free-living bacteria produce certain stimulatory metabolites that enhance plant development and growth, particularly when associated with AM fungi (Azcon 1993; Ahmad 1995). Within microbial species, the wide genetic variation explains the high potential of the micro-organisms to adapt to different environments. Therefore, it is necessary to develop specific host-strain combinations of both bacteria and AM fungi with high effectiveness under a wide range of experimental conditions (Medina et al. 2003).

Thus, these suggestions may be taken into account while offering the AM fungus-based bio-inoculants for sustainable plant production.

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