Mater lis

Low-melting-point agarose Hot plate Microscope slides Tissue samples

Equipment for microscopy using Nomarski optics Procedure

1. Prepare a 3% (w/v) solution of low-melting-point agarose in water.

2. Keep the liquid agarose solution on a hot plate.

3. Dip a microscope slide into the molten agarose.

4. Quickly lay the tissue on the agarose-covered slide.

5. Chill in the refrigerator for a few minutes.

6. Remove tissue and view, without a coverslip, under Nomarski optics.

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