Fixative (prepare in a chemical fume hood): 4 g of paraformaldehyde in 50 ml of distilled HzO.
Stir on a hot plate and warm the solution to 65°C.
Add 6 ml of NaOH (10 m) and stir until the solution becomes clear.
Filter through Whatman filter paper. Add 10 ml of lOx PBS (see below for PBS recipe) Add 40 ml of distilled H20 Store for up to 1 day at 4°C. lOx PBS (1 liter)
NaCl 76 g
Na2HP04.7H20 18.8 g
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