Quantitative Gus Activity Assays

This protocol was contributed by Miguel Blazquez (Salk Institute). GUS activity can be accurately determined both in extracts and in intact plant Lssue us ng 4-methylumbellyferyl p-D-glucuronide (4-MUG) as a substrate (Jefferson et al. 1987). Upon hydrolysis by GUS, the fluo-rochrome 4-methyl umbelliferone (4-MU) is produced. Using excitation at 365 nm, and measuring emission at 455 nm, the amount of 4-MU produced can be quantified. Under these conditions, background fluorescence from the...

Basta Selection on Soil

The major advantage of Basta selection is that it can be performed on plants growing in soil and does not require the use of sterile techniques. Basta glufosinate ammonium can be purchased as a herbicide solution from garden supply stores. In the United States, it is sold as Finale by Scotts Company, which has a long-term supply agreement with the manufacturer, AgrEvo. It can also be ordered on-line http www.biconet. com lawn finale.html . The pure chemical can be ordered in the United States...