Essential oils were obtained from various commercial sources and each oil was analysed by GC using a Shimadzu GC 8A with a 50m X 0.32 mm OV101 column; the temperature program was set at 4°Cmin_1 from 100 to 230°C.
The percentage of all the components was calculated in each selected Retention Time interval of under 10min, 11—15min, 16—20min, 21—30min and 30+ min. The main components present in each RT interval was also determined. The essential oils were diluted in methanol (usually X 1,000) and 0.1-0.2 ml was applied to the tissue preparations in the organ bath giving a final dilution of X200,000 to X400,000 (a concentration of 2.5 X 10~6 to 5 X 10~6).
Pharmacological studies, carried out on guinea-pig ileum were contrasted against many practising aromatherapists' predictions of the effect of essential oils on the patient (alone or as mixtures).
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