Morphological and Biochemical Characterization

Morphological and biochemical characterization of 4-year-old micropropagated as well as callus-regenerated plants was reported by Nirmal Babu (1997) in comparison with conventionally propagated plants (see Table 4.6). Direct regenerated plants and callus-regenerated plants as separate groups when compared with conventionally propagated plants revealed a good amount of variation with regard to plant height, number of tillers and of leaves per plant, girth of rhizome, number of nodes per finger, internodal distance, yield per plant (see Figure 4.2a—d), dry recovery percentage, and oleoresin and fiber contents. Micropropagated and callus-regenerated plants have higher mean values with regard to plant height, number of tillers, number of nodes per finger, and yield per plant compared to controls, whereas they have lesser mean values with regard to number of leaves per plant and oleoresin and fiber contents. With regard to the width of rhizomes,

Figure 4.2 Somaclonal variation and microrhizomes in ginger: (a) bold rhizomes harvested from ginger somaclone (CR 1222); (b) comparison of rhizomes from TC plants with that of conventionally propagated plants; (c) tissue-cultured plants after second year of nursery in comparison with conventionally propagated plants; (d) variations in rhizome size, shape, and internodal length in micropropagated plants; (e) growth recovery of microrhizome-derived plants in comparison with conventionally propagated plants; (f) initial growth of microrhizome-derived plants in comparison with conventionally propagated plants; (g) microrhizomes from ginger tissue cultures; (h) rhizomes harvested from microrhizomes as planting material.

Figure 4.2 Somaclonal variation and microrhizomes in ginger: (a) bold rhizomes harvested from ginger somaclone (CR 1222); (b) comparison of rhizomes from TC plants with that of conventionally propagated plants; (c) tissue-cultured plants after second year of nursery in comparison with conventionally propagated plants; (d) variations in rhizome size, shape, and internodal length in micropropagated plants; (e) growth recovery of microrhizome-derived plants in comparison with conventionally propagated plants; (f) initial growth of microrhizome-derived plants in comparison with conventionally propagated plants; (g) microrhizomes from ginger tissue cultures; (h) rhizomes harvested from microrhizomes as planting material.

Table 4.6 Morphological characters, yield, and quality attributes of promising somaclones in ginger

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