Photoautotrophically

Contents

1. Introduction

2. List of plant species successfully micropropagatedphotoautotrophically

3. References

Key words: Forced ventilation, natural ventilation, nutrient medium, photoautotrophic micropropagation, supporting material.

The concept of photoautotrophic micropropagation is derived from the research that revealed relatively high photosynthetic ability of chlorophyllous cultures such as leafy explants, cotyledonary stage somatic embryos and plantlets in vitro. While studying the environmental conditions of tissue culture vessels containing leafy green plantlets of Caiathea, Spatihphyllum, Phirodendron imbe Nepenthes, Dracaena, Cymbidium, Limonium, Syngonium, Cordyline, Ficus lyrata, Fujiwara et al. (1987) found that in vitro plantlets could not fully achieve their photosynthetic ability during the light period because the CO2 concentration in the closed vessels were too low in most of the light periods. Therefore, they concluded that the tissue cultured plantlets can be grown photoautotrophically (to be able to photosynthesise) during and after the multiplication stage by improving the CO2 and light environments in the culture vessels. In the following year (1988) several studies were reported in which photoautotrophic micropropagation, that is, growing plantlets in vitro in sugar-free medium was achieved. First of all, Kozai et al. (1988) successfully cultured potato (Solanum tuberosum L.) plantlets in sugar-free medium with a goal to develop an automated mass propagation system for producing disease-free seed-potato tubers and disease-free potato plantlets. In another study, Strawberry (Fragaria x ananassa) plants have been grown successfully under photoautotrophic conditions by Kozai and Sekimoto (1988). Carnation (Dianthus

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