O

Figure 12. Coffee somatic embryos regenerated from leaf discs after 14 weeks of culture under low light (30 ^mol m-2 s-1) followed by 2 weeks under high light (100 ^mol m-2 s-1) (x05). B—D, 45-d-old plantlets developed from cotyledonary stage embryos under photoautotrophic conditions in a temporary root zone immersion (TRI) bioreactor (B, x0.2), a Magenta vessel (C, x0.7) and a modified RITA-bioreactor (D, x0.2). E—G, Stomata from the abaxial (lower) surface of the first true leaves of plantlets developed photoautotrophically in a TRI-bioreactor (E), a Magenta vessel (F) and a modified RITA-bioreactor (G). H and I, Individual plantlets immediately before transplanting ex vitro grown in a TRI-bioreactor (H) and a Magenta vessel (I). J—L, Root development of plantlets grown in a TRI-bioreactor (J), a Magenta vessel (K) and a modified RITA-bioreactor (L). M-O, On day 30 after transplanting plantlets previously grown in a TRI-bioreactor (M), a Magenta vessel (N) and a modified RITA-bioreactor (O) (after Afreen et al, 2002b).

Figure 12. Coffee somatic embryos regenerated from leaf discs after 14 weeks of culture under low light (30 ^mol m-2 s-1) followed by 2 weeks under high light (100 ^mol m-2 s-1) (x05). B—D, 45-d-old plantlets developed from cotyledonary stage embryos under photoautotrophic conditions in a temporary root zone immersion (TRI) bioreactor (B, x0.2), a Magenta vessel (C, x0.7) and a modified RITA-bioreactor (D, x0.2). E—G, Stomata from the abaxial (lower) surface of the first true leaves of plantlets developed photoautotrophically in a TRI-bioreactor (E), a Magenta vessel (F) and a modified RITA-bioreactor (G). H and I, Individual plantlets immediately before transplanting ex vitro grown in a TRI-bioreactor (H) and a Magenta vessel (I). J—L, Root development of plantlets grown in a TRI-bioreactor (J), a Magenta vessel (K) and a modified RITA-bioreactor (L). M-O, On day 30 after transplanting plantlets previously grown in a TRI-bioreactor (M), a Magenta vessel (N) and a modified RITA-bioreactor (O) (after Afreen et al, 2002b).

Was this article helpful?

0 0

Post a comment