sugar added to the medium. Narrowly defined, photoautotrophy is a nutritional type where living organisms grow without any additional exogenous organic components as nutrients. When defined in this narrow sense, media in photoautotrophic micropropagation should exclude all organic components. As in hydroponics, media for photoautotrophic culture consist exclusively of inorganic components. Vitamins, growth regulators, and gelling agents should not be added to the medium in photoautotrophic micropropagation. Instead of gelling agents, porous substances like vermiculite should be employed as supporting materials in photoautotrophic micropropagation. As will be discussed in later chapters, use of such supporting materials along with liquid nutrient solution under controlled environment has beneficial effects on the growth and development of in vitro plantlets.
ideally, photoautotrophic micropropagation should be segregated from sugar-free micropropagation. However, in this chapter, while we will define photoautotrophy as the plant nutritional type where only endogenous carbohydrate is used as the energy source, for all practical purposes photoautotrophic micropropagation refers to micropropagation with no sugar added to the medium. Sugars and other carbohydrates may be significant components of agar and other gelling agents, but perhaps it is reasonable not to consider it as an exogenous carbohydrate source in the practical definition of photoautotrophic micropropagation.
Cultures without photosynthetically actve organs
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