General Characteristics Of In Vitro Microclimate

Plant microclimate refers to the climate in the immediate vicinity of a plant or surrounding the plant inside the culture vessel. Plant microclimate is of primary importance to the plant and differs from the culture room environment. In photomixotrophic micropropagation, the in vitro plant microclimate has unique characteristics compared with the ex vitro or the greenhouse environment, whereas in photoautotrophic micropropagation the in vitro plant microclimate resembles that of the ex vitro or...

Greenhouse

Increase in yearly productivity per floor area of the closed system is about 10 times that of a standard greenhouse for many kinds of transplants, as explained below and Table 14. 1) The ratio of planting area to floor area is usually 0.8 in the greenhouse, while it is 2.0 to 3.0 in the closed system with use of the multi-shelves (4 or 5 shelves). Thus, planting area per floor area of the closed system is about 2-3 times that of the greenhouse. On the other hand, use of multi-shelves is not...

Percent Survival During Acclimatization Ex Vitro

Percent survival of calla lily plantlets during the ex vitro acclimatization was 95 in the PAM and 60 in the PMM, and that of China fir was 95 in the PAM-0, 97 in the PAM-1, and 16 in the PMM. The percent survival was about 6 times higher in the PAM0 and the PAM1 than in the PMM. The lower percent survival in the PMM was probably due to the malfunction of stomata in leaves (Aitken-Christie et al., 1995) and callus formation at the base of the nodal cuttings. Callus formation has been considered...

Introduction

A 'closed plant production system' or simply a 'closed system' has been commercialized in Japan since 2002, largely based upon the research at Chiba University, for production of tomato, cucumber, eggplant, lettuce and spinach transplants from seeds. Commercial productions of herbs, leafy vegetables, bedding plants such as pansy, medicinal plants, and orchid plants are now under trial in Japan. The closed system is defined as a warehouse-like structure covered with opaque thermal insulators, in...

Forced Ventilation Systems With Large Culture Vessels

Micropropagation Lab

As described above, the method of using gas permeable filter discs attached to the lid or the sidewalls of the culture vessel or increasing the gaseous concentrations in the culture room is a simple way to effectively increase the CO2 concentration during the photoperiod and decrease the relative humidity and ethylene concentration inside a culture vessel. However, the CO2 concentration and other gaseous concentrations in the culture vessel with natural ventilation are interrelated with a...

Photoautotrophic Culture And Somatic Embryogenesis

In the process of photosynthesis, plants use light to convert atmospheric carbon dioxide into carbohydrate i.e. sucrose. Sucrose is one of the major end products of photosynthesis. In the conventional plant micropropagation, sucrose is generally supplied to the growth medium and absorbed by plants through roots. The exogenous supply of sucrose makes the plant incompetent for the process of photosynthesis and thus the plant becomes completely or partly dependent on supplied sucrose for the...