Effect of Other Additives on Antimicrobial Activity

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Other commonly used excipients were examined for their effect on the anti-microbial activity of tea tree oil. Simple 0.5% aqueous solutions, solubilised with polyoxyl 35 castor oil, were

Tea tree oil concentration 0.1%

Fails some bacteria

Fails Candida and A. niger

Passes bacteria

Passes Candida

Fails A. niger

Result (combined BP/USP test)

again used. The materials examined included EDTA, oil of thyme, propylene glycol and butylene glycol. All materials were examined in a range of concentrations, and it was found that none of the materials examined had any observable effect on the anti-microbial activity of the oil.

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