Enzymology of Nitrogen Assimilation in Mycorrhiza

IFTIKHAR AHMAD and JOHAN A. HELLEBUST

Centre for Plant Biotechnology, Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada

I. Introduction 181

II. Pure culture manipulation of mycorrhizal fungi for studies of nitrogen metabolism 184

A. Fungal cultures 184

B. Nitrogen utilization 185

III. Isolation and characterization of enzyme activities 185

A. Isolation 185

B. Enzyme characterization and assay procedures 186

IV. Pathways of ammonium assimilation 194

V. Induction of anabolic and catabolic pathways 195

VI. Extracellular enzymes 198

VII. Discussion 199

References 200

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