Summary

Echinacea species have been successfully regenerated from several explants, hypocotyl (Coker and Camper, 2000; Choffe et al., 2000a), cotyledon, petiole (Choffe et al., 2000a), and shoot-tips (Harbage, 2001). Explants successfully used for liquid cell-suspension cultures include callus tissue, leaf, and stem (Wagner et al., 1988; Schollhorn et al., 1993). Root tissue has been successfully transformed by Agrobacterium to produce hairy root cultures and callus (Trypsteen et al., 1991). Potential uses for cultured material includes year-round plant availability, material for mass production of specific clonal lines or repopulation of endangered species, a source of virus-free plant material, as well as a source of material for transformation experiments. Cultured material may also be used as a model system for studying metabolic functions as well as to manipulate metabolic pathways and the production of metabolites. This cultured material may be used for mass production of secondary products (i.e., arabinorhamnogalactan [Schollhorn et al., 1993], heteroxylan, and fucogalactoxyloglucan [Proksch et al., 1987]).

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