(i) Set up a calibration curve in five tubes: dilute 0.4 mg/mL cholesterol solution with chloroform:methanol (2:1) to prepare solutions 0.2, 0.4, 0.6 and 0.8 pM. Label tubes from 1 to 5. Then, add 0.5 mL of 0, 0.2, 0.4, 0.6 and 0.8 pM cholesterol to tubes 1, 2, 3, 4 and 5, respectively.

(ii) Set up your experimental assay tubes and prepare tubes 6 and 7 adding 0.25 and 0.50 mL of a plant extract, respectively. Complete volume of tube 6 to 0.5 mL with chloroform:methanol (2:1).

(iii) Add 5 mL of Lieberman-Buchard reagent to each tube.

(iv) Cap the test tubes, swirl and place in a water bath at 35Ā°C for 10 minutes.

(v) Measure the absorbance at 550 nm.

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Aromatherapy Ambiance

Aromatherapy Ambiance

Aromatherapy, a word often associated with calm, sweet smelling and relaxing surroundings. Made famous for its mostly relaxing indulgentĀ  feature, using aromatherapy has also been known to be related to have medicinal qualities.

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