Procedure

(i) Set up a calibration curve in five tubes: dilute 0.18 mg/mL glucose solution with distilled water to prepare solutions 60, 120, 190 and 250 pM. Label tubes from 1 to 5. Then, add 0.8 mL of 0, 60, 120, 190 and 250 pM glucose to tubes 1, 2, 3, 4 and 5, respectively.

(ii) Set up your experimental assay tubes and prepare tubes 6 and 7 adding 0.4 and 0.8 mL of the plant extract, respectively. Complete volume of tube 6 to 0.8 mL with distilled water.

(iii) Add 0.04 mL of 80% phenol to each tube and swirl. Add 2 mL of H2SO4 concentrated. Cover the mouth of each tube with a glass marble and put the tubes in a boiling water bath for 20 minutes. Let the tubes cool to room temperature.

(iv) Read absorbance at 490 nm using a 1 cm path length (1 mL) microcuvette.

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Aromatherapy Ambiance

Aromatherapy Ambiance

Aromatherapy, a word often associated with calm, sweet smelling and relaxing surroundings. Made famous for its mostly relaxing indulgent  feature, using aromatherapy has also been known to be related to have medicinal qualities.

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