(i) Set up a calibration curve in five tubes: dilute 0.18 mg/mL glucose solution with distilled water to prepare solutions 0, 0.1, 0.2, 0.3 and 0.4 mM. Label tubes from 1 to 5. Then, add 0.5 mL of 0, 0.1, 0.2, 0.3 and 0.4 mM glucose to tube 1, 2, 3, 4 and 5, respectively.

(ii) Set up your experimental assay tubes and prepare tubes 6 and 7 adding 0.25 and 0.50 mL of a plant extract, respectively. Complete volume of tube 6 to 0.5 mL with distilled water.

(iii) Add 0.5 mL of Somogyi reagent to each tube. Cover the mouth of each tube with a glass marble and put the tubes in a boiling water bath for

15 minutes. Let cool to room temperature. Add 0.5 mL of Nelson reagent to each tube and swirl.

(iv) Add 1 mL of distilled water to each tube and vortex.

(v) Read absorbance at 520 nm using a 1 cm path length (1 mL) microcuvette.

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Aromatherapy Ambiance

Aromatherapy Ambiance

Aromatherapy, a word often associated with calm, sweet smelling and relaxing surroundings. Made famous for its mostly relaxing indulgent  feature, using aromatherapy has also been known to be related to have medicinal qualities.

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