Procedure II

Extract fresh leaf tissue of a test plant by maceration with 20 volumes of cold isopropanol to deactivate the hydrolytic enzymes. Re-extract the residue with a mixture of chloroform-methanol (2:1, v/v). Grind the seeds to a coarse powder and extract it with chloroform-methanol (2:1, v/v) or with light petroleum. If lipids are tightly bound to the seed tissue, use the chloroform-ethanol-water (20:9.5:0.5) mixture for their extraction. The extracted material must be stored at 4Ā°C to avoid oxidation or in the presence of an antioxidant (e.g. 0.005% butylated hydroxy toluene, BHT).

10.3 Alkanes and Related Lipids

Alkanes are long-chain hydrocarbons distributed in the waxy coating film on the surface of leaves and fruits in plants . They are constituents of waxes. These with cutin, constitute the hydrophobic cuticle which limits water loss, controls gaseous exchange and participates in the protection against pathogenic agents. Waxes are a mixture of hydrocarbons, free and hydroxylated aliphatic acids, aliphatic alcohols, aliphatic aldehydes and aliphatic ketones, b-diketones and esters. They can also contain terpenoids and flavonoids.

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Aromatherapy Ambiance

Aromatherapy Ambiance

Aromatherapy, a word often associated with calm, sweet smelling and relaxing surroundings. Made famous for its mostly relaxing indulgentĀ  feature, using aromatherapy has also been known to be related to have medicinal qualities.

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