Extract ground, pulverized or freeze-dried plant material with a non-polar organic solvent (hexane, pentane, petroleum ether, or dichloromethane). The hydrophilic compounds including lignans, can then subsequently be extracted from the residue with a polar solvent such as acetone, methanol, or ethanol. The addition of 5-10% of water to the solvent will facilitate solvent penetration and favours the extraction of more polar compounds such as lignan glycosides. The process can be accelerated using elevated temperatures and pressures for short time periods of extraction.
Furthermore, lipids and terpenoids can be removed easily from oils by solid phase extraction (SPE) on C18 cartridges.
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