Step 1. Graph the calibration curve relating absorbance at 595 nm to BSA concentration. Fit a line function to the measured data points applying regression analysis with r2 > 0.80 (refer Calculations in Experiment 1).
Step 2. Calculate total protein concentration (C) in the plant extract expressed as BSA equivalent concentration. Consider absorbance at 595 nm (A595) from experimental tubes and extinction coefficient (e) obtained from linear regression: C (pg/mL) = (A595 x e) x 2 (tube 6) or C (pg/mL) = A595 x e (tube 7)
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