Step 1: Calculate the amount of a phenolic compound (APhc) extracted with neutral EDTA, considering concentration of phenolic compound (Cphc) determined after HPLC analysis and total volume of methanol fraction (Vm6oh):
Step 2: Express content of a phenolic compound extracted with EDTA (Cedta) per g of dry soil:
CEDTA = APhc (^mol)/dry weight (g) For example, if Aphc is 100 ^mol and dry weight is 20 g, then
Step 3: Calculate reversibly bound phenolic acids weakly sorbed on soil particles (Crb), subtracting the amount of phenolic compounds extracted with water (Cw) from that extracted with EDTA (Cedta):
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