Oh

Vanillin

Vanilloyl CoA

Figure 6.9 Possible biosynthetic pathway of vanillin formation from isoeugenol.

4-Hydroxy-3-methoxyphenyl-/?-hydroxypropionyl CoA W NAD

I * NADH CO-CH2-COSCoA

ocho

4-Hydroxy-3-methoxybenzoyl acetyl CoA

novel compounds. Though the amounts of vanilla flavoured metabolites is relatively low in view of the commercialization of these processes directly, the information generated on biosynthetic pathways and enzymes can be used to express at high levels using metabolic engineering approaches.

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