Chamomile Flowers Copaene Guaiazulene

zones

Guaiazulene: a red to reddish-violet zone

A red to reddish-violet zone (chamazulene)

Bornyl acetate: a yellowish-brown to grayish-green zone

A brown zone (en-yne-dicycloether)

Levomenol: a reddish-violet to bluish-violet zone

A reddish-violet to bluish-violet zone (levomenol) A brownish zone

Reference solution Test solution

Reference solution Test solution

B. Examine the chromatograms obtained in the test for chromatographic profile.

Results: The characteristic peaks corresponding to levomenol and to chamazulene in the chromato-gram obtained with the test solution are similar in retention time to those in the chromatogram obtained with the reference solution.

Chromatographic profile: Gas chromatography (2.2.28): use the normalization procedure. Test solution: Dissolve 20 jl of the oil to be examined in cyclohexane R and dilute to 5.0 ml with the same solvent.

Reference solution: Dissolve 20 jl of levomenol R, 5 mg of chamazulene R, and 6 mg of guaiazulene R in cyclohexane R and dilute to 5.0 ml with the same solvent.

Column:

• Material: Fused silica

• Size: l = 30 m (a film thickness of 1 jm may be used) to 60 m (a film thickness of 0.2 jm may be used), 0 = 0.25-0.53 mm; when using a column longer than 30 m, an adjustment of the temperature program may be necessary.

• Stationary phase: Macrogol 20,000 R

Carrier gas: Helium for chromatography R Flow rate: 1-2 ml/min Split ratio: 1:100

Temperature:

Column 0-40 70 ^ 230

40-50 230

Injection port 250

Detector 250

Detection: Flame ionization

Injection: 1.0 |l

Elution order: Order indicated in the composition of the reference solution. Record the retention times of these substances.

Relative retention: With reference to chamazulene (retention time = about 34.4 min): p-farnesene = about 0.5; bisabolol oxide B = about 0.8; bisabolone = about 0.87; levomenol = about 0.9; bisabolol oxide A = about 1.02.

System suitability: Reference solution: Resolution: Minimum of 1.5 between the peaks due to chamazulene and to guaiazulene.

Using the retention times determined from the chromatogram obtained with the reference solution, locate levomenol and chamazulene in the chromatogram obtained with the test solution; locate bisabolol oxides (bisabolol oxide B, bisabolone, and bisabolol oxide A) using Figures 1836.-1 and 1836.-2 (disregard the peak due to cyclohexane). The chromatogram obtained with the test solution does not show a peak with the retention time of guaiazulene.

Determine the percentage content of the components. The limits are within the following ranges:

Bisabolol oxides

Levomenol

Chamazulene

Total of bisabolol oxides and levomenol

Matricaria Oil Rich in Matricaria Oil Rich in

Bisabolol Oxides (%) Levomenol (%)

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