Highpressure liquid chromatography HPLC

For details, see El Rassi -1995) - Several forms of HPLC are useful for the resolution of mono- and oligosaccharides. In particular, a form of anion-exchange chromatography developed by Dionex Inc. enables excellent separation of all the common monosaccharides and numerous diverse oligosaccharides. The analytes are readily quantified with a pulsed ampero-metric detector if it has been calibrated for the compounds under investigation. Samples require more cleaning up than for PC. Radioactivity monitors designed for HPLC eluates are not sensitive enough (compared with auto-radiography or scintillation counting in vials) to be useful unless unusually high levels of radiolabelling are employed.

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