Osmotically induced Exocytosis and Endocytosis in Guard Cell Protoplasts

Investigation of Exocytosis and Endocytosis by Patch-clamp Capacitance Measurements Osmotically driven and pressure-driven changes in surface area of guard cell protoplasts have been investigated extensively by patch-clamp capacitance measurements. This technique allows the examination of exocytosis and endocytosis in single living protoplasts. Recordings can be performed with a resolution that is high enough to detect fusion and fission of vesicles with a diameter as low as 60 nm and a...