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(10)

(20)

(15)

Values summarize data from Table 1 in Strickland etal. (1998). Percent protection is calculated according to Formula 2. It should be noted that due to printer's errors, there are multiple shift errors in this table as originally published. The statistical analysis of this data is complex and the interested reader is advised to consult Table 2 in the original publication.

Notes

Values summarize data from Table 1 in Strickland etal. (1998). Percent protection is calculated according to Formula 2. It should be noted that due to printer's errors, there are multiple shift errors in this table as originally published. The statistical analysis of this data is complex and the interested reader is advised to consult Table 2 in the original publication.

4 ± 1% galactose, means ± SEM, analysis of 15 different isolations of polysaccharide from separate ARF lots) which is in accord with the sugar analyses in the older literature. Thus, in chemical terms we are dealing with the same polysaccharide with which everyone else is dealing. In physical terms, however, it is obvious that this material is substantially undegraded. We found (Strickland etal, 1998) that when fresh Process A gel was first harvested, it was biologically inactive in protecting the CHS immune response against UV injury. Table 12.12 illustrates the composite results from three preparations analysed over a period. Without addition of exogenous ' cellulase' (Table 12.12, ' None' column) there was a marginally significant treatment effect (Prep A, p = 0.026, Prep B, p = 0.006, Prep C, p>0.05) although there was a significant ' time' effect (Prep A, p = 0.0001, Prep B, p = 0.003, Prep C, p > 0.0015). Thus, although there was very little biological activity, something happened over time that was far greater than could be accounted for by experiment to experiment variation.

The surprising absence of therapeutic effect with extremely clean, extremely fresh gel was also seen in the classic phagocytic cell activation BRM assay with aloe. Figure 12.5 illustrates experiments by Dr Sheffield at the University of Wisconsin using materials we supplied. ARF Process A gel, in the absence of cellulase' (0 Cellulase) had only a meagre ability to activate macrophages. There was no significant activity at 1 ^g/ml, marginal activity at 10 ^g/ml, and substantial activity only at 100 ^g/ml. These results, and other measurements of activities traditionally ascribed to aloe extracts suggested that the ultra-clean Process A gel with the major polysaccharide in the native form was missing a major factor necessary to activate the extracts. Cellulase' treatment is necessary to activate native A. barbadensis gel for certain biological activities. We suspected that it was only as the Process A aloe gel was stored over time that it became biologically active (Table 12.12, 'None' column; '4 Weeks', 21 ±5% Protection; '5 Weeks', 32 + 10%) although with prolonged storage, the protective activity eventually decayed

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Aloe and Your Health

Aloe and Your Health

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