As a result of the problems arising from these previously described assays, there came a need for a more precise estimation of the individual chemicals in the exudate, both at the qualitative level to identify and confirm raw material and at the quantitative level to evaluate particular compounds with the view to determining quality and dosage.
An early report of separation of aloe compounds, followed by spectrophotometry determination, used a column of magnesia (magnesium oxide) and celite (diatomaceous earth) (1:3) with quantification by absorbance at 440 nm, although only the anthra-quinone aglycones and acid hydrolysates of glycosides were examined (Brody etal, 1950). Later, separation on a perlon (nylon) powder column eluted with aqueous methanol was used (Hörhammer etal, 1959). A summary of these earlier chromatographic and spectrophotometric studies is given by Böhme and Bertram (1955).
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