Arf

Notes a A & C are feedstock manufacturers. B was supplied by consumer product manufacturer and is known to be neither A nor B. D was supplied by manufacturer's representative and is neither A, B, nor C. b Conductivity of a 0.58 to 0.62 g/dl solution at ambient laboratory temperature. Results for maltodextrin standard are the means for determinations done on four separate occasions.

Results for ARF Process A Standard Samples are the means ± SEM for analyses done on twelve different Standard Samples. c Values expressed are the % of solids that are alcohol precipitable hexose. Results for ARF Process A Standard Samples are the means ± SEM for analyses done on twelve different Standard Samples.

d Conducted according to the IASC protocol. 'E Peak' is graded as normal in quantity, low, very low but still detectable or absent.

e 10g of powder were dialysed exhaustively using Spectraphor #1 tubing and deionized water. Samples were dialysed for a minimum of three times against at least five volumes of water. In all cases the conductivity of the final dialysate was under 100 jl Siemens. The data are expressed as percentage retained material (mass/mass). Results for ARF Standard Samples are the means ± SEM for analyses done on fifteen fractionations. f Determined by the phenol sulfuric acid assay for total hexose (Dubois Assay). Results for hexose content of ARF Standard Samples, high molecular weight fraction are the means ± SEM

for analyses done on ten different fractionations. In the case of the low MW fraction, 13 isolations. g Solutions of isolated polysaccharide, at a concentration of 1 mg/ml were scanned at wavelengths from 200 nm to 800 nm. Data are expressed as OD280nm. (Mean ± SEM of eight determinations from eight fractionations).

h Retained material was hydrolysed in 6N constant boiling HCl for 10 to 20 minutes at 120 °C. After neutralization, ratio of monosaccharides determined by HPLC on a Dionex PA-1 column with a pulsed amphoteric detector. ARF Standard Sample means are for 15 determinations on separate isolates. Means for commercial materials are for the four samples consistent with gel. i Determined by the Neocuprione assay for reducing sugar (Nelson Assay). Note: the reducing sugar content of the retained fraction (high molecular weight material) was 5.3 ± 0.3% (Mean ± SEM). This value was based on five fractionations of four ARF Process A Standard Samples of gel. For these Standard Samples the respective value for total hexose content of the low molecular weight fraction was 15.8 ± 1.5%. j Whole leaf preparation, polysacchride sugar composition is within normal limits when compared to a limited number of ARF WLE Standard Samples. Statistical values for the three samples alleged to be whole leaf are: glucose, 28 ± 17%; mannose, 48 ± 16%; galactose 14 ± 4%; coefficient of varience, 67%.

04 b

C Table 8.12B Characteristics of Commercial Powdered Maltodextrin Materials Alleged to be 'Aloe vera'.

5 No. Sourcea Unfractionated material Material greater than Material less than L 10,000 MW 10,000 MW

Was this article helpful?

0 0
Aloe and Your Health

Aloe and Your Health

This іѕ а health guide, whісh саn hеlр people knоw hоw tо mаkе uѕе оf Aloe vera tо improve оvеrаll health аnd deal wіth mаnу kinds оf diseases.

Get My Free Ebook


Post a comment